Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
Identifieur interne : 004571 ( Main/Exploration ); précédent : 004570; suivant : 004572Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
Auteurs : Maofeng Qiu [République populaire de Chine] ; Jin Wang ; Hongxia Wang ; Zeliang Chen ; Erhei Dai ; Zhaobiao Guo ; Xiaoyi Wang ; Xin Pang ; Baoxing Fan ; Jie Wen ; Jian Wang ; Ruifu YangSource :
- Clinical and diagnostic laboratory immunology [ 1071-412X ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Anticorps antiviraux, Nucléocapside.
- diagnostic : Syndrome de détresse respiratoire de l'adulte.
- isolement et purification : Coronavirus.
- normes : Test ELISA.
- Coronavirus, Humains, Sensibilité et spécificité, Sites de fixation, Test ELISA, Tests sérologiques.
English descriptors
- KwdEn :
- Antibodies, Viral (analysis), Binding Sites, Coronavirus (chemistry), Coronavirus (isolation & purification), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Humans, Nucleocapsid (analysis), Respiratory Distress Syndrome, Adult (diagnosis), Sensitivity and Specificity, Serologic Tests (methods).
- MESH :
- chemical , analysis : Antibodies, Viral.
- analysis : Nucleocapsid.
- chemistry : Coronavirus.
- diagnosis : Respiratory Distress Syndrome, Adult.
- isolation & purification : Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- standards : Enzyme-Linked Immunosorbent Assay.
- Binding Sites, Humans, Sensitivity and Specificity.
Abstract
Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.
DOI: 10.1128/CDLI.12.3.474-476.2005
PubMed: 15753261
Affiliations:
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Le document en format XML
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for serodiagnosis and epidemiological study of SARS was developed.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name><name sortKey="Fan, Baoxing" sort="Fan, Baoxing" uniqKey="Fan B" first="Baoxing" last="Fan">Baoxing Fan</name><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name><name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Qiu, Maofeng" sort="Qiu, Maofeng" uniqKey="Qiu M" first="Maofeng" last="Qiu">Maofeng Qiu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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